Membrane proteins, lipids and detergents: not just a soap opera.
Identifieur interne : 001C18 ( Main/Exploration ); précédent : 001C17; suivant : 001C19Membrane proteins, lipids and detergents: not just a soap opera.
Auteurs : Annela M. Seddon [Royaume-Uni] ; Paul Curnow ; Paula J. BoothSource :
- Biochimica et biophysica acta [ 0006-3002 ] ; 2004.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Detergents, Membrane Lipids, Membrane Proteins.
- chemical , isolation & purification : Membrane Proteins.
- Crystallization, Liposomes.
Abstract
Studying membrane proteins represents a major challenge in protein biochemistry, with one of the major difficulties being the problems encountered when working outside the natural lipid environment. In vitro studies such as crystallization are reliant on the successful solubilization or reconstitution of membrane proteins, which generally involves the careful selection of solubilizing detergents and mixed lipid/detergent systems. This review will concentrate on the methods currently available for efficient reconstitution and solubilization of membrane proteins through the use of detergent micelles, mixed lipid/detergent micelles and bicelles or liposomes. We focus on the relevant molecular properties of the detergents and lipids that aid understanding of these processes. A significant barrier to membrane protein research is retaining the stability and function of the protein during solubilization, reconstitution and crystallization. We highlight some of the lessons learnt from studies of membrane protein folding in vitro and give an overview of the role that lipids can play in stabilizing the proteins.
DOI: 10.1016/j.bbamem.2004.04.011
PubMed: 15519311
Affiliations:
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Le document en format XML
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Booth</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="doi">10.1016/j.bbamem.2004.04.011</idno><idno type="RBID">pubmed:15519311</idno><idno type="pmid">15519311</idno><idno type="wicri:Area/PubMed/Corpus">000538</idno><idno type="wicri:Area/PubMed/Curation">000538</idno><idno type="wicri:Area/PubMed/Checkpoint">000523</idno><idno type="wicri:Area/Ncbi/Merge">000314</idno><idno type="wicri:Area/Ncbi/Curation">000314</idno><idno type="wicri:Area/Ncbi/Checkpoint">000314</idno><idno type="wicri:doubleKey">0006-3002:2004:Seddon A:membrane:proteins:lipids</idno><idno type="wicri:Area/Main/Merge">001C68</idno><idno type="wicri:Area/Main/Curation">001C18</idno><idno type="wicri:Area/Main/Exploration">001C18</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Membrane proteins, lipids and detergents: not just a soap opera.</title><author><name sortKey="Seddon, Annela M" sort="Seddon, Annela M" uniqKey="Seddon A" first="Annela M" last="Seddon">Annela M. Seddon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD</wicri:regionArea><wicri:noRegion>Bristol BS8 1TD</wicri:noRegion></affiliation></author><author><name sortKey="Curnow, Paul" sort="Curnow, Paul" uniqKey="Curnow P" first="Paul" last="Curnow">Paul Curnow</name></author><author><name sortKey="Booth, Paula J" sort="Booth, Paula J" uniqKey="Booth P" first="Paula J" last="Booth">Paula J. Booth</name></author></analytic><series><title level="j">Biochimica et biophysica acta</title><idno type="ISSN">0006-3002</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Crystallization</term><term>Detergents (chemistry)</term><term>Liposomes</term><term>Membrane Lipids (chemistry)</term><term>Membrane Proteins (chemistry)</term><term>Membrane Proteins (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Detergents</term><term>Membrane Lipids</term><term>Membrane Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Membrane Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Crystallization</term><term>Liposomes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Studying membrane proteins represents a major challenge in protein biochemistry, with one of the major difficulties being the problems encountered when working outside the natural lipid environment. In vitro studies such as crystallization are reliant on the successful solubilization or reconstitution of membrane proteins, which generally involves the careful selection of solubilizing detergents and mixed lipid/detergent systems. This review will concentrate on the methods currently available for efficient reconstitution and solubilization of membrane proteins through the use of detergent micelles, mixed lipid/detergent micelles and bicelles or liposomes. We focus on the relevant molecular properties of the detergents and lipids that aid understanding of these processes. A significant barrier to membrane protein research is retaining the stability and function of the protein during solubilization, reconstitution and crystallization. We highlight some of the lessons learnt from studies of membrane protein folding in vitro and give an overview of the role that lipids can play in stabilizing the proteins.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><noCountry><name sortKey="Booth, Paula J" sort="Booth, Paula J" uniqKey="Booth P" first="Paula J" last="Booth">Paula J. Booth</name><name sortKey="Curnow, Paul" sort="Curnow, Paul" uniqKey="Curnow P" first="Paul" last="Curnow">Paul Curnow</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Seddon, Annela M" sort="Seddon, Annela M" uniqKey="Seddon A" first="Annela M" last="Seddon">Annela M. Seddon</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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